6E64

Crystal structure of malaria transmission-blocking antibody 85RF45.1

Summary for 6E64

• Entry DOI: 10.2210/pdb6e64/pdb
• Descriptor: 85RF45.1 Fab heavy chain, 85RF45.1 Fab light chain (2 entities in total)
• Functional Keywords: malaria, transmission-blocking, antibody, immune system
• Biological source: Rattus norvegicus; More
• Total number of polymer chains: 4
• Total formula weight: 94601.45

Authors

Kundu, P.,Semesi, A.,Julien, J.P. (deposition date: 2018-07-23, release date: 2018-11-28, Last modification date: 2024-10-23)

Primary citation

Kundu, P.,Semesi, A.,Jore, M.M.,Morin, M.J.,Price, V.L.,Liang, A.,Li, J.,Miura, K.,Sauerwein, R.W.,King, C.R.,Julien, J.P.
Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45.
Nat Commun, 9:4458-4458, 2018

PubMed Abstract: Interventions that can block the transmission of malaria-causing Plasmodium falciparum (Pf) between the human host and Anopheles vector have the potential to reduce the incidence of malaria. Pfs48/45 is a gametocyte surface protein critical for parasite development and transmission, and its targeting by monoclonal antibody (mAb) 85RF45.1 leads to the potent reduction of parasite transmission. Here, we reveal how the Pfs48/45 6C domain adopts a (SAG1)-related-sequence (SRS) fold. We structurally delineate potent epitope I and show how mAb 85RF45.1 recognizes an electronegative surface with nanomolar affinity. Analysis of Pfs48/45 sequences reveals that polymorphisms are rare for residues involved at the binding interface. Humanization of rat-derived mAb 85RF45.1 conserved the mode of recognition and activity of the parental antibody, while also improving its thermostability. Our work has implications for the development of transmission-blocking interventions, both through improving vaccine designs and the testing of passive delivery of mAbs in humans.

PubMed: 30367064
DOI: 10.1038/s41467-018-06742-9

Experimental method

X-RAY DIFFRACTION (3.15 Å)