6E5X

Crystal structure of Ebola virus VP30 C-terminus/RBBP6 peptide complex

Summary for 6E5X

• Entry DOI: 10.2210/pdb6e5x/pdb
• Descriptor: Minor nucleoprotein VP30, E3 ubiquitin-protein ligase RBBP6, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: viral protein
• Biological source: Zaire ebolavirus (strain Kikwit-95) (ZEBOV); More
• Total number of polymer chains: 2
• Total formula weight: 16060.77

Authors

Liu, D.,Small, G.I.,Leung, D.W.,Amarasinghe, G.K. (deposition date: 2018-07-23, release date: 2018-12-12, Last modification date: 2023-10-11)

Primary citation

Batra, J.,Hultquist, J.F.,Liu, D.,Shtanko, O.,Von Dollen, J.,Satkamp, L.,Jang, G.M.,Luthra, P.,Schwarz, T.M.,Small, G.I.,Arnett, E.,Anantpadma, M.,Reyes, A.,Leung, D.W.,Kaake, R.,Haas, P.,Schmidt, C.B.,Schlesinger, L.S.,LaCount, D.J.,Davey, R.A.,Amarasinghe, G.K.,Basler, C.F.,Krogan, N.J.
Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication.
Cell, 175:1917-1930.e13, 2018

PubMed Abstract: Ebola virus (EBOV) infection often results in fatal illness in humans, yet little is known about how EBOV usurps host pathways during infection. To address this, we used affinity tag-purification mass spectrometry (AP-MS) to generate an EBOV-host protein-protein interaction (PPI) map. We uncovered 194 high-confidence EBOV-human PPIs, including one between the viral transcription regulator VP30 and the host ubiquitin ligase RBBP6. Domain mapping identified a 23 amino acid region within RBBP6 that binds to VP30. A crystal structure of the VP30-RBBP6 peptide complex revealed that RBBP6 mimics the viral nucleoprotein (NP) binding to the same interface of VP30. Knockdown of endogenous RBBP6 stimulated viral transcription and increased EBOV replication, whereas overexpression of either RBBP6 or the peptide strongly inhibited both. These results demonstrate the therapeutic potential of biologics that target this interface and identify additional PPIs that may be leveraged for novel therapeutic strategies.

PubMed: 30550789
DOI: 10.1016/j.cell.2018.08.044

Experimental method

X-RAY DIFFRACTION (1.5 Å)