6E4V

The Crystal Structure of FhuE from E. coli in complex with its substrate Coprogen

6E4V の概要

• エントリーDOI: 10.2210/pdb6e4v/pdb
• 分子名称: FhuE receptor, octyl beta-D-glucopyranoside, COPROGEN, ... (4 entities in total)
• 機能のキーワード: tonb-dependent transporter, coprogen, gram-negative bacteria, outer membrane, iron transport, transport protein
• 由来する生物種: Escherichia coli BW25113
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 79757.26

構造登録者

Grinter, R.,Lithgow, T. (登録日: 2018-07-18, 公開日: 2019-04-10, 最終更新日: 2023-10-11)

主引用文献

Grinter, R.,Lithgow, T.
Determination of the molecular basis for coprogen import by Gram-negative bacteria.
Iucrj, 6:401-411, 2019

PubMed Abstract: In order to survive in mixed microbial communities, some species of fungi secrete coprogens, siderophores that facilitate capture of the scarce nutrient iron. The TonB-dependent transporter FhuE is integrated in the outer membrane of Gram-negative bacteria and has been reported to scavenge these fungally produced coprogens. In this work, an strain was engineered that is dependent solely on FhuE for its access to siderophore-sequestered iron. Using this tool, it is shown that while FhuE is highly active in the import of coprogens, it has some level of promiscuity, acting as a low-affinity transporter for related siderophores. The crystal structure of FhuE in complex with coprogen was determined, providing a structural basis to explain this selective promiscuity. The structural data, in combination with functional analysis, presented in this work show that FhuE has evolved to specifically engage with planar siderophores. A potential evolutionary driver, and a critical consequence of this selectivity, is that it allows FhuE to exclude antibiotics that mimic nonplanar hydroxamate siderophores: these toxic molecules could otherwise cross the outer membrane barrier through a Trojan horse mechanism.

PubMed: 31098021
DOI: 10.1107/S2052252519002926

実験手法

X-RAY DIFFRACTION (2 Å)