6E49

Pif1 peptide bound to PCNA trimer

「6B8I」から置き換えられました

6E49 の概要

• エントリーDOI: 10.2210/pdb6e49/pdb
• 分子名称: Proliferating cell nuclear antigen, ATP-dependent DNA helicase PIF1 (3 entities in total)
• 機能のキーワード: complex, pcna, pif1 peptide, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 97782.46

構造登録者

Buzovetsky, O.,Kwon, Y.,Pham, N.T.,Kim, C.,Ira, G.,Sung, P.,Xiong, Y. (登録日: 2018-07-17, 公開日: 2018-08-22, 最終更新日: 2024-03-13)

主引用文献

Buzovetsky, O.,Kwon, Y.,Pham, N.T.,Kim, C.,Ira, G.,Sung, P.,Xiong, Y.
Role of the Pif1-PCNA Complex in Pol delta-Dependent Strand Displacement DNA Synthesis and Break-Induced Replication.
Cell Rep, 21:1707-1714, 2017

PubMed Abstract: The S. cerevisiae Pif1 helicase functions with DNA polymerase (Pol) δ in DNA synthesis during break-induced replication (BIR), a conserved pathway responsible for replication fork repair and telomere recombination. Pif1 interacts with the DNA polymerase processivity clamp PCNA, but the functional significance of the Pif1-PCNA complex remains to be elucidated. Here, we solve the crystal structure of PCNA in complex with a non-canonical PCNA-interacting motif in Pif1. The structure guides the construction of a Pif1 mutant that is deficient in PCNA interaction. This mutation impairs the ability of Pif1 to enhance DNA strand displacement synthesis by Pol δ in vitro and also the efficiency of BIR in cells. These results provide insights into the role of the Pif1-PCNA-Pol δ ensemble during DNA break repair by homologous recombination.

PubMed: 29141206
DOI: 10.1016/j.celrep.2017.10.079

実験手法

X-RAY DIFFRACTION (2.9 Å)