6DY6

Mn(II)-bound structure of the engineered cyt cb562 variant, CH2E

6DY6 の概要

• エントリーDOI: 10.2210/pdb6dy6/pdb
• 関連するPDBエントリー: 6DY4
• 分子名称: Soluble cytochrome b562, MANGANESE (II) ION, HEME C, ... (5 entities in total)
• 機能のキーワード: designed protein, 4-helix bundle, electron transport, metal binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25199.65

構造登録者

Tezcan, F.A.,Rittle, J. (登録日: 2018-07-01, 公開日: 2019-04-24, 最終更新日: 2024-11-13)

主引用文献

Rittle, J.,Field, M.J.,Green, M.T.,Tezcan, F.A.
An efficient, step-economical strategy for the design of functional metalloproteins.
Nat.Chem., 11:434-441, 2019

PubMed Abstract: The bottom-up design and construction of functional metalloproteins remains a formidable task in biomolecular design. Although numerous strategies have been used to create new metalloproteins, pre-existing knowledge of the tertiary and quaternary protein structure is often required to generate suitable platforms for robust metal coordination and activity. Here we report an alternative and easily implemented approach (metal active sites by covalent tethering or MASCoT) in which folded protein building blocks are linked by a single disulfide bond to create diverse metal coordination environments within evolutionarily naive protein-protein interfaces. Metalloproteins generated using this strategy uniformly bind a wide array of first-row transition metal ions (Mn, Fe, Co, Ni, Cu, Zn and vanadyl) with physiologically relevant thermodynamic affinities (dissociation constants ranging from 700 nM for Mn to 50 fM for Cu). MASCoT readily affords coordinatively unsaturated metal centres-including a penta-His-coordinated non-haem Fe site-and well-defined binding pockets that can accommodate modifications and enable coordination of exogenous ligands such as nitric oxide to the interfacial metal centre.

PubMed: 30778140
DOI: 10.1038/s41557-019-0218-9

実験手法

X-RAY DIFFRACTION (1.8 Å)