6DTL

Mitogen-activated protein kinase 6

6DTL の概要

• エントリーDOI: 10.2210/pdb6dtl/pdb
• 分子名称: Mitogen-activated protein kinase 6 (2 entities in total)
• 機能のキーワード: kinase, plant protein, transferase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84304.41

構造登録者

Ruble, J. (登録日: 2018-06-17, 公開日: 2019-05-15, 最終更新日: 2024-03-13)

主引用文献

Putarjunan, A.,Ruble, J.,Srivastava, A.,Zhao, C.,Rychel, A.L.,Hofstetter, A.K.,Tang, X.,Zhu, J.K.,Tama, F.,Zheng, N.,Torii, K.U.
Bipartite anchoring of SCREAM enforces stomatal initiation by coupling MAP kinases to SPEECHLESS.
Nat.Plants, 5:742-754, 2019

PubMed Abstract: Cell fate in eukaryotes is controlled by mitogen-activated protein kinases (MAPKs) that translate external cues into cellular responses. In plants, two MAPKs-MPK3 and MPK6-regulate diverse processes of development, environmental response and immunity. However, the mechanism that bridges these shared signalling components with a specific target remains unresolved. Focusing on the development of stomata-epidermal valves that are essential for gas exchange and transpiration-here, we report that the basic helix-loop-helix protein SCREAM functions as a scaffold that recruits MPK3/6 to downregulate SPEECHLESS, a transcription factor that initiates stomatal cell lineages. SCREAM directly binds to MPK3/6 through an evolutionarily conserved, yet unconventional, bipartite motif. Mutations in this motif abrogate association, phosphorylation and degradation of SCREAM, unmask hidden non-redundancies between MPK3 and MPK6, and result in uncontrolled stomatal differentiation. Structural analyses of MPK6 with a resolution of 2.75 Å showed bipartite binding of SCREAM to MPK6 that is distinct from an upstream MAPKK. Our findings elucidate, at the atomic resolution, the mechanism that directly links extrinsic signals to transcriptional reprogramming during the establishment of stomatal cell fate, and highlight a unique substrate-binding mode adopted by plant MAPKs.

PubMed: 31235876
DOI: 10.1038/s41477-019-0440-x

実験手法

X-RAY DIFFRACTION (2.753 Å)