6DT0

Cryo-EM structure of a mitochondrial calcium uniporter

6DT0 の概要

• エントリーDOI: 10.2210/pdb6dt0/pdb
• EMDBエントリー: 8911
• 分子名称: Mitochondrial calcium uniporter, CALCIUM ION (2 entities in total)
• 機能のキーワード: mitochondrial calcium uniporter, calcium-selective ion channel, calcium uptake, uniporter, transport protein
• 由来する生物種: Neurospora crassa
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 211832.55

構造登録者

Yoo, J.,Wu, M.,Yin, Y.,Herzik, M.A.J.,Lander, G.C.,Lee, S.-Y. (登録日: 2018-06-14, 公開日: 2018-07-11, 最終更新日: 2024-03-13)

主引用文献

Yoo, J.,Wu, M.,Yin, Y.,Herzik Jr., M.A.,Lander, G.C.,Lee, S.Y.
Cryo-EM structure of a mitochondrial calcium uniporter.
Science, 361:506-511, 2018

PubMed Abstract: Calcium transport plays an important role in regulating mitochondrial physiology and pathophysiology. The mitochondrial calcium uniporter (MCU) is a calcium-selective ion channel that is the primary mediator for calcium uptake into the mitochondrial matrix. Here, we present the cryo-electron microscopy structure of the full-length MCU from to an overall resolution of ~3.7 angstroms. Our structure reveals a tetrameric architecture, with the soluble and transmembrane domains adopting different symmetric arrangements within the channel. The conserved W-D-Φ-Φ-E-P-V-T-Y sequence motif of MCU pore forms a selectivity filter comprising two acidic rings separated by one helical turn along the central axis of the channel pore. The structure combined with mutagenesis gives insight into the basis of calcium recognition.

PubMed: 29954988
DOI: 10.1126/science.aar4056

実験手法

ELECTRON MICROSCOPY (3.7 Å)