6DRV

Beta-galactosidase

6DRV の概要

• エントリーDOI: 10.2210/pdb6drv/pdb
• EMDBエントリー: 8908
• 分子名称: Beta-galactosidase (1 entity in total)
• 機能のキーワード: cryo-em, amazon web services, relion, hydrolase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 466409.94

構造登録者

Cianfrocco, M.A.,Lahiri, I.,DiMaio, F.,Leschziner, A.E. (登録日: 2018-06-13, 公開日: 2018-07-11, 最終更新日: 2024-10-23)

主引用文献

Cianfrocco, M.A.,Lahiri, I.,DiMaio, F.,Leschziner, A.E.
cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.
J. Struct. Biol., 203:230-235, 2018

PubMed Abstract: Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2 Å structure of ß-galactosidase in ∼55 h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud.

PubMed: 29864529
DOI: 10.1016/j.jsb.2018.05.014

実験手法

ELECTRON MICROSCOPY (2.2 Å)