6DOT

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 40 s at 21 C

6DOT の概要

• エントリーDOI: 10.2210/pdb6dot/pdb
• Group deposition: Cleavage of RNA by B. Halodurans Ribonuclease H1 (G_1001001)
• 分子名称: Ribonuclease H, 5'-R(*AP*CP*AP*U)-3' portion of uncleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3', 5'-R(P*CP*G)-3' portion of uncleaved RNA 5'-R(*AP*CP*AP*UP*CP*G)-3', ... (10 entities in total)
• 機能のキーワード: protein-rna-dna complex, double helix, rna hydrolysis, in crystallo catalysis, metal dependent catalysis, monovalent cations, divalent cations, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna
• 由来する生物種: Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 20305.10

構造登録者

Samara, N.L.,Yang, W. (登録日: 2018-06-09, 公開日: 2018-08-15, 最終更新日: 2023-10-11)

• 主引用文献: Samara, N.L.,Yang, W.; Cation trafficking propels RNA hydrolysis.; Nat. Struct. Mol. Biol., 25:715-721, 2018; PubMed: 30076410; DOI: 10.1038/s41594-018-0099-4

実験手法

X-RAY DIFFRACTION (1.423 Å)