6DNW

Sequence Requirements of the Listeria innocua prophage attP site

6DNW の概要

• エントリーDOI: 10.2210/pdb6dnw/pdb
• 分子名称: Putative integrase [Bacteriophage A118], DNA (26-MER), ZINC ION, ... (5 entities in total)
• 機能のキーワード: site-specific recombination, phage integrase, serine integrase, attachment site, integration, specificity, dna binding protein
• 由来する生物種: Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 53966.05

構造登録者

Li, H.,Sharp, R.,Rutherford, K.,Gupta, K.,Van Duyne, G.D. (登録日: 2018-06-08, 公開日: 2018-10-03, 最終更新日: 2023-10-11)

主引用文献

Li, H.,Sharp, R.,Rutherford, K.,Gupta, K.,Van Duyne, G.D.
Serine Integrase attP Binding and Specificity.
J. Mol. Biol., 430:4401-4418, 2018

PubMed Abstract: Serine integrases catalyze the site-specific insertion of viral DNA into a host's genome. The minimal requirements and irreversible nature of this integration reaction have led to the use of serine integrases in applications ranging from bacterial memory storage devices to gene therapy. Our understanding of how the integrase proteins recognize the viral (attP) and host (attB) attachment sites is limited, with structural data available for only a Listeria integrase C-terminal domain (CTD) bound to an attP half-site. Here we report quantitative binding and saturation mutagenesis analyses for the Listeria innocua prophage attP site and a new 2.8-Å crystal structure of the CTD•attP half site. We find that Int binds with high affinity to attP (6.9 nM), but the Int CTD binds to attP half-sites with only 7- to 10-fold lower affinity, supporting the idea that free energy is expended to open an Int dimer for attP binding. Despite the 50-bp Int-attP interaction surface, only 20 residues are sensitive to mutagenesis, and of these, only 6 require a specific residue for efficient Int binding and integration activity. One of the integrase DNA-binding domains, the recombinase domain, appears to be primarily non-specific. Several substitutions result in an improved attP site, indicating that higher-efficiency attachment sites can be obtained through site engineering. These findings advance our understanding of serine integrase function and provide important data for efforts towards engineering this family of enzymes for a variety of biotechnology applications.

PubMed: 30227134
DOI: 10.1016/j.jmb.2018.09.007

実験手法

X-RAY DIFFRACTION (2.849 Å)