6DK4
Crystal structure of Campylobacter jejuni peroxide stress regulator
Summary for 6DK4
| Entry DOI | 10.2210/pdb6dk4/pdb |
| Descriptor | Ferric uptake regulation protein, MANGANESE (II) ION, ZINC ION, ... (5 entities in total) |
| Functional Keywords | perr, ferric uptake regulator, campylobacter jejuni, peroxide stress, metal transport |
| Biological source | Campylobacter jejuni |
| Total number of polymer chains | 2 |
| Total formula weight | 34208.25 |
| Authors | Sarvan, S.,Brunzelle, J.S.,Couture, J.F. (deposition date: 2018-05-28, release date: 2018-06-13, Last modification date: 2024-03-06) |
| Primary citation | Sarvan, S.,Charih, F.,Butcher, J.,Brunzelle, J.S.,Stintzi, A.,Couture, J.F. Crystal structure of Campylobacter jejuni peroxide regulator. FEBS Lett., 592:2351-2360, 2018 Cited by PubMed Abstract: In Campylobacter jejuni (Cj), the metal-cofactored peroxide response regulator (PerR) transcription factor allows C. jejuni to respond to oxidative stresses. The crystal structure of the metalated form of CjPerR shows that the protein folds as an asymmetric dimer displaying structural differences in the orientation of its DNA-binding domain. Comparative analysis shows that such asymmetry is a conserved feature among crystallized PerR proteins, and mutational analysis reveals that residues found in the first α-helix of CjPerR contribute to DNA binding. These studies present the structure of CjPerR protein and highlight structural heterogeneity in the orientation of the metalated PerR DNA-binding domain which may underlie the ability of PerR to recognize DNA, control gene expression, and contribute to bacterial pathogenesis. PubMed: 29856899DOI: 10.1002/1873-3468.13120 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.71 Å) |
Structure validation
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