6DK4

Crystal structure of Campylobacter jejuni peroxide stress regulator

6DK4 の概要

• エントリーDOI: 10.2210/pdb6dk4/pdb
• 分子名称: Ferric uptake regulation protein, MANGANESE (II) ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: perr, ferric uptake regulator, campylobacter jejuni, peroxide stress, metal transport
• 由来する生物種: Campylobacter jejuni
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34208.25

構造登録者

Sarvan, S.,Brunzelle, J.S.,Couture, J.F. (登録日: 2018-05-28, 公開日: 2018-06-13, 最終更新日: 2024-03-06)

主引用文献

Sarvan, S.,Charih, F.,Butcher, J.,Brunzelle, J.S.,Stintzi, A.,Couture, J.F.
Crystal structure of Campylobacter jejuni peroxide regulator.
FEBS Lett., 592:2351-2360, 2018

PubMed Abstract: In Campylobacter jejuni (Cj), the metal-cofactored peroxide response regulator (PerR) transcription factor allows C. jejuni to respond to oxidative stresses. The crystal structure of the metalated form of CjPerR shows that the protein folds as an asymmetric dimer displaying structural differences in the orientation of its DNA-binding domain. Comparative analysis shows that such asymmetry is a conserved feature among crystallized PerR proteins, and mutational analysis reveals that residues found in the first α-helix of CjPerR contribute to DNA binding. These studies present the structure of CjPerR protein and highlight structural heterogeneity in the orientation of the metalated PerR DNA-binding domain which may underlie the ability of PerR to recognize DNA, control gene expression, and contribute to bacterial pathogenesis.

PubMed: 29856899
DOI: 10.1002/1873-3468.13120

実験手法

X-RAY DIFFRACTION (2.71 Å)