6DID

HIV Env BG505 SOSIP with polyclonal Fabs from immunized rabbit #3417 post-boost#1

6DID の概要

• エントリーDOI: 10.2210/pdb6did/pdb
• EMDBエントリー: 7896
• 分子名称: Envelope glycoprotein gp160, alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: hiv, antibodies, antibody, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 378243.01

構造登録者

Turner, H.L.,Cottrell, C.A.,Oyen, D.,Wilson, I.A.,Ward, A.B. (登録日: 2018-05-23, 公開日: 2018-09-05, 最終更新日: 2024-11-20)

主引用文献

Bianchi, M.,Turner, H.L.,Nogal, B.,Cottrell, C.A.,Oyen, D.,Pauthner, M.,Bastidas, R.,Nedellec, R.,McCoy, L.E.,Wilson, I.A.,Burton, D.R.,Ward, A.B.,Hangartner, L.
Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization.
Immunity, 49:288-300.e8, 2018

PubMed Abstract: Characterizing polyclonal antibody responses via currently available methods is inherently complex and difficult. Mapping epitopes in an immune response is typically incomplete, which creates a barrier to fully understanding the humoral response to antigens and hinders rational vaccine design efforts. Here, we describe a method of characterizing polyclonal responses by using electron microscopy, and we applied this method to the immunization of rabbits with an HIV-1 envelope glycoprotein vaccine candidate, BG505 SOSIP.664. We detected known epitopes within the polyclonal sera and revealed how antibody responses evolved during the prime-boosting strategy to ultimately result in a neutralizing antibody response. We uncovered previously unidentified epitopes, including an epitope proximal to one recognized by human broadly neutralizing antibodies as well as potentially distracting non-neutralizing epitopes. Our method provides an efficient and semiquantitative map of epitopes that are targeted in a polyclonal antibody response and should be of widespread utility in vaccine and infection studies.

PubMed: 30097292
DOI: 10.1016/j.immuni.2018.07.009

実験手法

ELECTRON MICROSCOPY (4.71 Å)