6DGM

Streptococcus pyogenes phosphoglycerol transferase GacH in complex with sn-glycerol-1-phosphate

6DGM の概要

• エントリーDOI: 10.2210/pdb6dgm/pdb
• 関連するPDBエントリー: 5U9Z
• 分子名称: Phosphoglycerol transferase GacH, MANGANESE (II) ION, SN-GLYCEROL-1-PHOSPHATE, ... (5 entities in total)
• 機能のキーワード: group a streptococcus (gas), lancefield group a carbohydrate, glycero-1-phosphate, l-glycerol 1-phosphate, d-glycerol-3-phosphate, transferase
• 由来する生物種: Streptococcus pyogenes MGAS5005
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87030.87

構造登録者

Edgar, R.J.,Korotkova, N.,Korotkov, K.V. (登録日: 2018-05-17, 公開日: 2018-06-06, 最終更新日: 2023-10-11)

主引用文献

Edgar, R.J.,van Hensbergen, V.P.,Ruda, A.,Turner, A.G.,Deng, P.,Le Breton, Y.,El-Sayed, N.M.,Belew, A.T.,McIver, K.S.,McEwan, A.G.,Morris, A.J.,Lambeau, G.,Walker, M.J.,Rush, J.S.,Korotkov, K.V.,Widmalm, G.,van Sorge, N.M.,Korotkova, N.
Discovery of glycerol phosphate modification on streptococcal rhamnose polysaccharides.
Nat.Chem.Biol., 15:463-471, 2019

PubMed Abstract: Cell wall glycopolymers on the surface of Gram-positive bacteria are fundamental to bacterial physiology and infection biology. Here we identify gacH, a gene in the Streptococcus pyogenes group A carbohydrate (GAC) biosynthetic cluster, in two independent transposon library screens for its ability to confer resistance to zinc and susceptibility to the bactericidal enzyme human group IIA-secreted phospholipase A. Subsequent structural and phylogenetic analysis of the GacH extracellular domain revealed that GacH represents an alternative class of glycerol phosphate transferase. We detected the presence of glycerol phosphate in the GAC, as well as the serotype c carbohydrate from Streptococcus mutans, which depended on the presence of the respective gacH homologs. Finally, nuclear magnetic resonance analysis of GAC confirmed that glycerol phosphate is attached to approximately 25% of the GAC N-acetylglucosamine side-chains at the C6 hydroxyl group. This previously unrecognized structural modification impacts host-pathogen interaction and has implications for vaccine design.

PubMed: 30936502
DOI: 10.1038/s41589-019-0251-4

実験手法

X-RAY DIFFRACTION (1.49 Å)