6DFG

BG505 MD39 SOSIP trimer in complex with mature BG18 fragment antigen binding

6DFG の概要

• エントリーDOI: 10.2210/pdb6dfg/pdb
• 関連するPDBエントリー: 6DFH
• EMDBエントリー: 7875 7876
• 分子名称: Envelope glycoprotein gp160, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: hiv-1, hiv envelope, sosip, germline targeting, antibody, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 384555.97

構造登録者

Ozorowski, G.,Steichen, J.M.,Schief, W.R.,Ward, A.B. (登録日: 2018-05-14, 公開日: 2019-11-06, 最終更新日: 2024-10-16)

主引用文献

Steichen, J.M.,Lin, Y.C.,Havenar-Daughton, C.,Pecetta, S.,Ozorowski, G.,Willis, J.R.,Toy, L.,Sok, D.,Liguori, A.,Kratochvil, S.,Torres, J.L.,Kalyuzhniy, O.,Melzi, E.,Kulp, D.W.,Raemisch, S.,Hu, X.,Bernard, S.M.,Georgeson, E.,Phelps, N.,Adachi, Y.,Kubitz, M.,Landais, E.,Umotoy, J.,Robinson, A.,Briney, B.,Wilson, I.A.,Burton, D.R.,Ward, A.B.,Crotty, S.,Batista, F.D.,Schief, W.R.
A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses.
Science, 366:-, 2019

PubMed Abstract: Vaccine induction of broadly neutralizing antibodies (bnAbs) to HIV remains a major challenge. Germline-targeting immunogens hold promise for initiating the induction of certain bnAb classes; yet for most bnAbs, a strong dependence on antibody heavy chain complementarity-determining region 3 (HCDR3) is a major barrier. Exploiting ultradeep human antibody sequencing data, we identified a diverse set of potential antibody precursors for a bnAb with dominant HCDR3 contacts. We then developed HIV envelope trimer-based immunogens that primed responses from rare bnAb-precursor B cells in a mouse model and bound a range of potential bnAb-precursor human naïve B cells in ex vivo screens. Our repertoire-guided germline-targeting approach provides a framework for priming the induction of many HIV bnAbs and could be applied to most HCDR3-dominant antibodies from other pathogens.

PubMed: 31672916
DOI: 10.1126/science.aax4380

実験手法

ELECTRON MICROSCOPY (4.42 Å)