6DFD

Crystal structure of CNNM3 cyclic nucleotide-binding homology domain

6DFD の概要

• エントリーDOI: 10.2210/pdb6dfd/pdb
• 関連するPDBエントリー: 6DJ3
• 分子名称: Metal transporter CNNM3 (2 entities in total)
• 機能のキーワード: beta-barrel fold, cyclic nucleotide-binding homology domain, magnesium transporter, metal transport
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58802.57

構造登録者

Kozlov, G.,Gehring, K. (登録日: 2018-05-14, 公開日: 2018-10-31, 最終更新日: 2024-10-16)

主引用文献

Chen, Y.S.,Kozlov, G.,Fakih, R.,Funato, Y.,Miki, H.,Gehring, K.
The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+efflux activity.
J. Biol. Chem., 293:19998-20007, 2018

PubMed Abstract: Proteins of the cyclin M family (CNNMs; also called ancient conserved domain proteins, or ACDPs) are represented by four integral membrane proteins that have been proposed to function as Mg transporters. CNNMs are associated with a number of genetic diseases affecting ion movement and cancer via their association with highly oncogenic phosphatases of regenerating liver (PRLs). Structurally, CNNMs contain an N-terminal extracellular domain, a transmembrane domain (DUF21), and a large cytosolic region containing a cystathionine-β-synthase (CBS) domain and a putative cyclic nucleotide-binding homology (CNBH) domain. Although the CBS domain has been extensively characterized, little is known about the CNBH domain. Here, we determined the first crystal structures of the CNBH domains of CNNM2 and CNNM3 at 2.6 and 1.9 Å resolutions. Contrary to expectation, these domains did not bind cyclic nucleotides, but mediated dimerization both in crystals and in solution. Analytical ultracentrifugation experiments revealed an inverse correlation between the propensity of the CNBH domains to dimerize and the ability of CNNMs to mediate Mg efflux. CNBH domains from active family members were observed as both dimers and monomers, whereas the inactive member, CNNM3, was observed only as a dimer. Mutational analysis revealed that the CNBH domain was required for Mg efflux activity of CNNM4. This work provides a structural basis for understanding the function of CNNM proteins in Mg transport and associated diseases.

PubMed: 30341174
DOI: 10.1074/jbc.RA118.005672

実験手法

X-RAY DIFFRACTION (1.901 Å)