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6DET

The crystal structure of Tv2483 bound to L-arginine

Summary for 6DET
Entry DOI10.2210/pdb6det/pdb
DescriptorTv2483, ARGININE (3 entities in total)
Functional Keywordsabc transporter, amino-acid-binding protein, ligand-binding protein, transport protein
Biological sourceTreponema vincentii
Total number of polymer chains1
Total formula weight30372.44
Authors
Brautigam, C.A.,Norgard, M.V. (deposition date: 2018-05-13, release date: 2019-03-20, Last modification date: 2024-03-13)
Primary citationDeka, R.K.,Liu, W.Z.,Tso, S.C.,Norgard, M.V.,Brautigam, C.A.
Biophysical insights into a highly selective l-arginine-binding lipoprotein of a pathogenic treponeme.
Protein Sci., 27:2037-2050, 2018
Cited by
PubMed Abstract: Biophysical and biochemical studies on the lipoproteins and other periplasmic proteins from the spirochetal species Treponema pallidum have yielded numerous insights into the functioning of the organism's peculiar membrane organization, its nutritional requirements, and intermediary metabolism. However, not all T. pallidum proteins have proven to be amenable to biophysical studies. One such recalcitrant protein is Tp0309, a putative polar-amino-acid-binding protein of an ABC transporter system. To gain further information on its possible function, a homolog of the protein from the related species T. vincentii was used as a surrogate. This protein, Tv2483, was crystallized, resulting in the determination of its crystal structure at a resolution of 1.75 Å. The protein has a typical fold for a ligand-binding protein, and a single molecule of l-arginine was bound between its two lobes. Differential scanning fluorimetry and isothermal titration calorimetry experiments confirmed that l-arginine bound to the protein with unusually high selectivity. However, further comparison to Tp0309 showed differences in key amino-acid-binding residues may impart an alternate specificity for the T. pallidum protein.
PubMed: 30242931
DOI: 10.1002/pro.3510
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.75 Å)
Structure validation

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