6DAM

Crystal structure of lanthanide-dependent methanol dehydrogenase XoxF from Methylomicrobium buryatense 5G

6DAM の概要

• エントリーDOI: 10.2210/pdb6dam/pdb
• 分子名称: Lanthanide-dependent methanol dehydrogenase XoxF, LANTHANUM (III) ION, PYRROLOQUINOLINE QUINONE, ... (5 entities in total)
• 機能のキーワード: dehydrogenase, pqq, methanol, xoxf, lanthanide, lanthanum, methanotroph, oxidoreductase
• 由来する生物種: Methylomicrobium buryatense 5G
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67770.81

構造登録者

Deng, Y.,Ro, S.Y.,Rosenzweig, A.C. (登録日: 2018-05-01, 公開日: 2018-09-05, 最終更新日: 2024-11-13)

主引用文献

Deng, Y.W.,Ro, S.Y.,Rosenzweig, A.C.
Structure and function of the lanthanide-dependent methanol dehydrogenase XoxF from the methanotroph Methylomicrobium buryatense 5GB1C.
J. Biol. Inorg. Chem., 23:1037-1047, 2018

PubMed Abstract: In methylotrophic bacteria, which use one-carbon (C1) compounds as a carbon source, methanol is oxidized by pyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenase (MDH) enzymes. Methylotrophic genomes generally encode two distinct MDHs, MxaF and XoxF. MxaF is a well-studied, calcium-dependent heterotetrameric enzyme whereas XoxF is a lanthanide-dependent homodimer. Recent studies suggest that XoxFs are likely the functional MDHs in many environments. In methanotrophs, methylotrophs that utilize methane, interactions between particulate methane monooxygenase (pMMO) and MxaF have been detected. To investigate the possibility of interactions between pMMO and XoxF, XoxF was isolated from the methanotroph Methylomicrobium buryatense 5GB1C (5G-XoxF). Purified 5G-XoxF exhibits a specific activity of 0.16 μmol DCPIP reduced min mg. The 1.85 Å resolution crystal structure reveals a La(III) ion in the active site, in contrast to the calcium ion in MxaF. The overall fold is similar to other MDH structures, but 5G-XoxF is a monomer in solution. An interaction between 5G-XoxF and its cognate pMMO was detected by biolayer interferometry, with a K value of 50 ± 17 μM. These results suggest an alternative model of MDH-pMMO association, in which a XoxF monomer may bind to pMMO, and underscore the potential importance of lanthanide-dependent MDHs in biological methane oxidation.

PubMed: 30132076
DOI: 10.1007/s00775-018-1604-2

実験手法

X-RAY DIFFRACTION (1.85 Å)