6D97

Structure of aldehyde dehydrogenase 12 (ALDH12) from Zea mays

6D97 の概要

• エントリーDOI: 10.2210/pdb6d97/pdb
• 分子名称: Aldehyde dehydrogenase 12, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total)
• 機能のキーワード: aldh12, rossmann fold, l-glutamate-gamma-semialdehyde dehydrogenase, oxidoreductase
• 由来する生物種: Zea mays (Maize)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 244518.44

構造登録者

Tanner, J.J.,Korasick, D.A.,Kopecny, D. (登録日: 2018-04-27, 公開日: 2019-01-09, 最終更新日: 2023-10-04)

主引用文献

Korasick, D.A.,Koncitikova, R.,Kopecna, M.,Hajkova, E.,Vigouroux, A.,Morera, S.,Becker, D.F.,Sebela, M.,Tanner, J.J.,Kopecny, D.
Structural and Biochemical Characterization of Aldehyde Dehydrogenase 12, the Last Enzyme of Proline Catabolism in Plants.
J. Mol. Biol., 431:576-592, 2019

PubMed Abstract: Heterokonts, Alveolata protists, green algae from Charophyta and Chlorophyta divisions, and all Embryophyta plants possess an aldehyde dehydrogenase (ALDH) gene named ALDH12. Here, we provide a biochemical characterization of two ALDH12 family members from the lower plant Physcomitrella patens and higher plant Zea mays. We show that ALDH12 encodes an NAD-dependent glutamate γ-semialdehyde dehydrogenase (GSALDH), which irreversibly converts glutamate γ-semialdehyde (GSAL), a mitochondrial intermediate of the proline and arginine catabolism, to glutamate. Sedimentation equilibrium and small-angle X-ray scattering analyses reveal that in solution both plant GSALDHs exist as equilibrium between a domain-swapped dimer and the dimer-of-dimers tetramer. Plant GSALDHs share very low-sequence identity with bacterial, fungal, and animal GSALDHs (classified as ALDH4), which are the closest related ALDH superfamily members. Nevertheless, the crystal structure of ZmALDH12 at 2.2-Å resolution  shows that nearly all key residues involved in the recognition of GSAL are identical to those in ALDH4, indicating a close functional relationship with ALDH4. Phylogenetic analysis suggests that the transition from ALDH4 to ALDH12 occurred during the evolution of the endosymbiotic plant ancestor, prior to the evolution of green algae and land plants. Finally, ALDH12 expression in maize and moss is downregulated in response to salt and drought stresses, possibly to maintain proline levels. Taken together, these results provide molecular insight into the biological roles of the plant ALDH12 family.

PubMed: 30580036
DOI: 10.1016/j.jmb.2018.12.010

実験手法

X-RAY DIFFRACTION (2.2 Å)