6D95

Ternary RsAgo Complex with Guide RNA Paired and Target DNA containing A8-A8' Non-Canonical Pair

6D95 の概要

• エントリーDOI: 10.2210/pdb6d95/pdb
• 関連するPDBエントリー: 6D8A 6D8F 6D8P 6D92
• 分子名称: Uncharacterized protein, RNA (5'-R(P*UP*UP*AP*CP*UP*GP*CP*AP*CP*AP*GP*GP*UP*GP*AP*CP*GP*A)-3'), DNA 24-Mer, ... (5 entities in total)
• 機能のキーワード: rhodobacter sphaerodes argounaute (rsago); guide rna; target dna; rna-dna heteroduplex; non-canonical base pairs and bulges, rna binding protein
• 由来する生物種: Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 196502.11

構造登録者

Liu, Y.,Esyunina, D.,Olovnikov, I.,Teplova, M.,Patel, D.J. (登録日: 2018-04-27, 公開日: 2018-08-08, 最終更新日: 2024-03-13)

主引用文献

Liu, Y.,Esyunina, D.,Olovnikov, I.,Teplova, M.,Kulbachinskiy, A.,Aravin, A.A.,Patel, D.J.
Accommodation of Helical Imperfections in Rhodobacter sphaeroides Argonaute Ternary Complexes with Guide RNA and Target DNA.
Cell Rep, 24:453-462, 2018

PubMed Abstract: Prokaryotic Argonaute (Ago) proteins were recently shown to target foreign genetic elements, thus making them a perfect model for studies of interference mechanisms. Here, we study interactions of Rhodobacter sphaeroides Ago (RsAgo) with guide RNA (gRNA) and fully complementary or imperfect target DNA (tDNA) using biochemical and structural approaches. We show that RsAgo can specifically recognize both the first nucleotide in gRNA and complementary nucleotide in tDNA, and both interactions contribute to nucleic acid binding. Non-canonical pairs and bulges on the target strand can be accommodated by RsAgo with minimal perturbation of the duplex but significantly reduce RsAgo affinity to tDNA. Surprisingly, mismatches between gRNA and tDNA induce dissociation of the guide-target duplex from RsAgo. Our results reveal plasticity in the ability of Ago proteins to accommodate helical imperfections, show how this might affect the efficiency of RNA silencing, and suggest a potential mechanism for guide release and Ago recycling.

PubMed: 29996105
DOI: 10.1016/j.celrep.2018.06.021

実験手法

X-RAY DIFFRACTION (1.85 Å)