6D90

Mammalian 80S ribosome with a double translocated CrPV-IRES, P-site tRNA and eRF1.

これはPDB形式変換不可エントリーです。

6D90 の概要

• エントリーDOI: 10.2210/pdb6d90/pdb
• EMDBエントリー: 7834
• 分子名称: Ribosomal protein L8, Ribosomal protein L11, eL13, ... (85 entities in total)
• 機能のキーワード: ribosome, mammalian, crpv ires
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 85
• 化学式量合計: 3465649.22

構造登録者

Pisareva, V.P.,Pisarev, A.V.,Fernandez, I.S. (登録日: 2018-04-27, 公開日: 2018-06-06, 最終更新日: 2024-12-25)

主引用文献

Pisareva, V.P.,Pisarev, A.V.,Fernandez, I.S.
Dual tRNA mimicry in the Cricket Paralysis Virus IRES uncovers an unexpected similarity with the Hepatitis C Virus IRES.
Elife, 7:-, 2018

PubMed Abstract: Co-opting the cellular machinery for protein production is a compulsory requirement for viruses. The Cricket Paralysis Virus employs an Internal Ribosomal Entry Site (CrPV-IRES) to express its structural genes in the late stage of infection. Ribosome hijacking is achieved by a sophisticated use of molecular mimicry to tRNA and mRNA, employed to manipulate intrinsically dynamic components of the ribosome. Binding and translocation through the ribosome is required for this IRES to initiate translation. We report two structures, solved by single particle electron cryo-microscopy (cryoEM), of a double translocated CrPV-IRES with aminoacyl-tRNA in the peptidyl site (P site) of the ribosome. CrPV-IRES adopts a previously unseen conformation, mimicking the acceptor stem of a canonical E site tRNA. The structures suggest a mechanism for the positioning of the first aminoacyl-tRNA shared with the distantly related Hepatitis C Virus IRES.

PubMed: 29856316
DOI: 10.7554/eLife.34062

実験手法

ELECTRON MICROSCOPY (3.2 Å)