6D57

Campylobacter jejuni ferric uptake regulator S1 metalated

6D57 の概要

• エントリーDOI: 10.2210/pdb6d57/pdb
• 分子名称: Ferric uptake regulation protein, GLYCEROL, FORMIC ACID, ... (5 entities in total)
• 機能のキーワード: fur, ferric uptake regulator, campylobacter jejuni, metalloregulator, metal transport
• 由来する生物種: Campylobacter jejuni
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37598.02

構造登録者

Sarvan, S.,Brunzelle, J.S.,Couture, J.F. (登録日: 2018-04-19, 公開日: 2018-05-23, 最終更新日: 2025-04-02)

主引用文献

Sarvan, S.,Charih, F.,Askoura, M.,Butcher, J.,Brunzelle, J.S.,Stintzi, A.,Couture, J.F.
Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators.
Sci Rep, 8:7140-7140, 2018

PubMed Abstract: Ferric uptake regulators (Fur) are a family of transcription factors coupling gene regulatory events to metal concentration. Recent evidence has expanded the mechanistic repertoires employed by Fur to activate or repress gene expression in the presence or absence of regulatory metals. However, the mechanistic basis underlying this extended repertoire has remained largely unexplored. In this study, we used an extensive set of mutations to demonstrate that Campylobacter jejuni Fur (CjFur) employs the same surface to positively and negatively control gene expression regardless of the presence or absence of metals. Moreover, the crystal structure determination of a CjFur devoid of any regulatory metals shows that subtle reorientation of the transcription factor DNA binding domain negatively impacts DNA binding, gene expression and gut colonization in chickens. Overall, these results highlight the versatility of the CjFur DNA binding domain in mediating all gene regulatory events controlled by the metalloregulator and that the full metalation of CjFur is critical to the Campylobacter jejuni life cycle in vivo.

PubMed: 29739988
DOI: 10.1038/s41598-018-25157-6

実験手法

X-RAY DIFFRACTION (1.81 Å)