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6D0H

ParT: Prs ADP-ribosylating toxin bound to cognate antitoxin ParS

6D0H の概要
エントリーDOI10.2210/pdb6d0h/pdb
分子名称ParT: COG5654 (RES domain) toxin, ParS: COG5642 (DUF2384) antitoxin, GLYCEROL, ... (4 entities in total)
機能のキーワードadp-ribosyltransferase, toxin-antitoxin complex, parst, toxin
由来する生物種Sphingobium sp. YBL2
詳細
タンパク質・核酸の鎖数4
化学式量合計49992.55
構造登録者
Piscotta, F.J.,Jeffrey, P.D.,Link, A.J. (登録日: 2018-04-10, 公開日: 2019-01-09, 最終更新日: 2024-03-13)
主引用文献Piscotta, F.J.,Jeffrey, P.D.,Link, A.J.
ParST is a widespread toxin-antitoxin module that targets nucleotide metabolism.
Proc. Natl. Acad. Sci. U.S.A., 116:826-834, 2019
Cited by
PubMed Abstract: Toxin-antitoxin (TA) systems interfere with essential cellular processes and are implicated in bacterial lifestyle adaptations such as persistence and the biofilm formation. Here, we present structural, biochemical, and functional data on an uncharacterized TA system, the COG5654-COG5642 pair. Bioinformatic analysis showed that this TA pair is found in 2,942 of the 16,286 distinct bacterial species in the RefSeq database. We solved a structure of the toxin bound to a fragment of the antitoxin to 1.50 Å. This structure suggested that the toxin is a mono-ADP-ribosyltransferase (mART). The toxin specifically modifies phosphoribosyl pyrophosphate synthetase (Prs), an essential enzyme in nucleotide biosynthesis conserved in all organisms. We propose renaming the toxin ParT for Prs ADP-ribosylating toxin and ParS for the cognate antitoxin. ParT is a unique example of an intracellular protein mART in bacteria and is the smallest known mART. This work demonstrates that TA systems can induce bacteriostasis through interference with nucleotide biosynthesis.
PubMed: 30598453
DOI: 10.1073/pnas.1814633116
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.5 Å)
構造検証レポート
Validation report summary of 6d0h
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-23に公開中

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