6D07

Crystal structure of the complex between human chromobox homolog 1 (CBX1) and H3K9me3 peptide

6D07 の概要

• エントリーDOI: 10.2210/pdb6d07/pdb
• 関連するPDBエントリー: 6D08
• 分子名称: Chromobox protein homolog 1, Histone H3.1, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: lysine modification, chromodomain, bump-hole, epigenetics, protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 16296.40

構造登録者

Arora, S.,Horne, W.S.,Islam, K. (登録日: 2018-04-10, 公開日: 2019-04-10, 最終更新日: 2023-10-04)

主引用文献

Arora, S.,Horne, W.S.,Islam, K.
Engineering Methyllysine Writers and Readers for Allele-Specific Regulation of Protein-Protein Interactions.
J.Am.Chem.Soc., 141:15466-15470, 2019

PubMed Abstract: Protein-protein interactions mediated by methyllysine are ubiquitous in biological systems. Specific perturbation of such interactions has remained a challenging endeavor. Herein, we describe an allele-specific strategy toward an engineered protein-protein interface orthogonal to the human proteome. We develop a methyltransferase (writer) variant that installs aryllysine moiety on histones that can only be recognized by an engineered chromodomain (reader). We establish biochemical integrity of the engineered interface, provide structural evidence for orthogonality and validate its applicability to identify transcriptional regulators. Our approach provides an unprecedented strategy for specific manipulation of the methyllysine interactome.

PubMed: 31518125
DOI: 10.1021/jacs.9b05725

実験手法

X-RAY DIFFRACTION (2.1 Å)