6CP0

SdcA in complex with the E2, UbcH5C

6CP0 の概要

• エントリーDOI: 10.2210/pdb6cp0/pdb
• 分子名称: SdcA, Ubiquitin-conjugating enzyme E2 D3 (3 entities in total)
• 機能のキーワード: complex, bacterial e3 ligase, e2, ligase
• 由来する生物種: Legionella pneumophila; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78881.00

構造登録者

Wasilko, D.J.,Huang, Q.,Mao, Y. (登録日: 2018-03-13, 公開日: 2018-08-01, 最終更新日: 2023-10-04)

主引用文献

Wasilko, D.J.,Huang, Q.,Mao, Y.
Insights into the ubiquitin transfer cascade catalyzed by theLegionellaeffector SidC.
Elife, 7:-, 2018

PubMed Abstract: The causative agent of Legionnaires' disease, , delivers more than 330 virulent effectors to its host to establish an intracellular membrane-bound organelle called the containing vacuole. Among the army of effectors, SidC and its paralog SdcA have been identified as novel bacterial ubiquitin (Ub) E3 ligases. To gain insight into the molecular mechanism of SidC/SdcA as Ub ligases, we determined the crystal structures of a binary complex of the N-terminal catalytic SNL domain of SdcA with its cognate E2 UbcH5C and a ternary complex consisting of the SNL domain of SidC with the Ub-linked E2 UbcH7. These two structures reveal the molecular determinants governing the Ub transfer cascade catalyzed by SidC. Together, our data support a common mechanism in the Ub transfer cascade in which the donor Ub is immobilized with its C-terminal tail locked in an extended conformation, priming the donor Ub for catalysis.

PubMed: 30015617
DOI: 10.7554/eLife.36154

実験手法

X-RAY DIFFRACTION (3.01 Å)