6CNW

STRUCTURE OF HUMANIZED SINGLE DOMAIN ANTIBODY SD84

6CNW の概要

• エントリーDOI: 10.2210/pdb6cnw/pdb
• 分子名称: humanized antibody SD84h, ACETATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: single domain antibody, humanization, influenza, hemagglutinin, immune system
• 由来する生物種: hybrid
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13166.55

構造登録者

Luo, J.,Obmolova, O. (登録日: 2018-03-09, 公開日: 2018-11-14, 最終更新日: 2024-10-23)

主引用文献

Laursen, N.S.,Friesen, R.H.E.,Zhu, X.,Jongeneelen, M.,Blokland, S.,Vermond, J.,van Eijgen, A.,Tang, C.,van Diepen, H.,Obmolova, G.,van der Neut Kolfschoten, M.,Zuijdgeest, D.,Straetemans, R.,Hoffman, R.M.B.,Nieusma, T.,Pallesen, J.,Turner, H.L.,Bernard, S.M.,Ward, A.B.,Luo, J.,Poon, L.L.M.,Tretiakova, A.P.,Wilson, J.M.,Limberis, M.P.,Vogels, R.,Brandenburg, B.,Kolkman, J.A.,Wilson, I.A.
Universal protection against influenza infection by a multidomain antibody to influenza hemagglutinin.
Science, 362:598-602, 2018

PubMed Abstract: Broadly neutralizing antibodies against highly variable pathogens have stimulated the design of vaccines and therapeutics. We report the use of diverse camelid single-domain antibodies to influenza virus hemagglutinin to generate multidomain antibodies with impressive breadth and potency. Multidomain antibody MD3606 protects mice against influenza A and B infection when administered intravenously or expressed locally from a recombinant adeno-associated virus vector. Crystal and single-particle electron microscopy structures of these antibodies with hemagglutinins from influenza A and B viruses reveal binding to highly conserved epitopes. Collectively, our findings demonstrate that multidomain antibodies targeting multiple epitopes exhibit enhanced virus cross-reactivity and potency. In combination with adeno-associated virus-mediated gene delivery, they may provide an effective strategy to prevent infection with influenza virus and other highly variable pathogens.

PubMed: 30385580
DOI: 10.1126/science.aaq0620

実験手法

X-RAY DIFFRACTION (0.92 Å)