6CK4

G96A mutant of the PRPP riboswitch from T. mathranii bound to ppGpp

6CK4 の概要

• エントリーDOI: 10.2210/pdb6ck4/pdb
• 分子名称: PRPP riboswitch, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: riboswitch, ykkc, prpp, ppgpp, rna
• 由来する生物種: Thermoanaerobacter mathranii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 155114.49

構造登録者

Reiss, C.W.,Knappenberger, A.J.,Strobel, S.A. (登録日: 2018-02-27, 公開日: 2018-06-20, 最終更新日: 2024-03-13)

主引用文献

Knappenberger, A.J.,Reiss, C.W.,Strobel, S.A.
Structures of two aptamers with differing ligand specificity reveal ruggedness in the functional landscape of RNA.
Elife, 7:-, 2018

PubMed Abstract: Two classes of riboswitches related to the guanidine-I riboswitch bind phosphoribosyl pyrophosphate (PRPP) and guanosine tetraphosphate (ppGpp). Here we report the co-crystal structure of the PRPP aptamer and its ligand. We also report the structure of the G96A point mutant that prefers ppGpp over PRPP with a dramatic 40,000-fold switch in specificity. The ends of the aptamer form a helix that is not present in the guanidine aptamer and is involved in the expression platform. In the mutant, the base of ppGpp replaces G96 in three-dimensional space. This disrupts the S-turn, which is a primary structural feature of the RNA motif. These dramatic differences in ligand specificity are achieved with minimal mutations. aptamers are therefore a prime example of an RNA fold with a rugged fitness landscape. The ease with which the aptamer acquires new specificity represents a striking case of evolvability in RNA.

PubMed: 29877798
DOI: 10.7554/eLife.36381

実験手法

X-RAY DIFFRACTION (3.097 Å)