6CIH

Crystal structure of a group II intron lariat in the post-catalytic state

6CIH の概要

• エントリーDOI: 10.2210/pdb6cih/pdb
• 分子名称: RNA (696-MER), RNA (5'-R(P*UP*GP*UP*UP*UP*AP*UP*UP*AP*AP*AP*AP*AP*C*-3'), IRIDIUM HEXAMMINE ION, ... (6 entities in total)
• 機能のキーワード: group ii intron, rna splicing, spliceosome, lariat, rna
• 由来する生物種: Pylaiella littoralis; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 219637.18

構造登録者

Chan, R.T.,Peters, J.K.,Robart, A.R.,Wiryaman, T.,Rajashankar, K.R.,Toor, N. (登録日: 2018-02-23, 公開日: 2018-11-21, 最終更新日: 2024-03-13)

主引用文献

Chan, R.T.,Peters, J.K.,Robart, A.R.,Wiryaman, T.,Rajashankar, K.R.,Toor, N.
Structural basis for the second step of group II intron splicing.
Nat Commun, 9:4676-4676, 2018

PubMed Abstract: The group II intron and the spliceosome share a common active site architecture and are thought to be evolutionarily related. Here we report the 3.7 Å crystal structure of a eukaryotic group II intron in the lariat-3' exon form, immediately preceding the second step of splicing, analogous to the spliceosomal P complex. This structure reveals the location of the intact 3' splice site within the catalytic core of the group II intron. The 3'-OH of the 5' exon is positioned in close proximity to the 3' splice site for nucleophilic attack and exon ligation. The active site undergoes conformational rearrangements with the catalytic triplex having different configurations before and after the second step of splicing. We describe a complete model for the second step of group II intron splicing that incorporates a dynamic catalytic triplex being responsible for creating the binding pocket for 3' splice site capture.

PubMed: 30410046
DOI: 10.1038/s41467-018-06678-0

実験手法

X-RAY DIFFRACTION (3.676 Å)