6CFX

Bosea sp GapR solved in the presence of DNA

6CFX の概要

• エントリーDOI: 10.2210/pdb6cfx/pdb
• 分子名称: UPF0335 protein ASE63_04290, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: caulobacter, dna binding, nap, dna twist, dna binding protein
• 由来する生物種: Bosea sp. Root381
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 36374.69

構造登録者

Schumacher, M.A. (登録日: 2018-02-18, 公開日: 2018-09-12, 最終更新日: 2023-10-04)

主引用文献

Guo, M.S.,Haakonsen, D.L.,Zeng, W.,Schumacher, M.A.,Laub, M.T.
A Bacterial Chromosome Structuring Protein Binds Overtwisted DNA to Stimulate Type II Topoisomerases and Enable DNA Replication.
Cell, 175:583-, 2018

PubMed Abstract: When DNA is unwound during replication, it becomes overtwisted and forms positive supercoils in front of the translocating DNA polymerase. Unless removed or dissipated, this superhelical tension can impede replication elongation. Topoisomerases, including gyrase and topoisomerase IV in bacteria, are required to relax positive supercoils ahead of DNA polymerase but may not be sufficient for replication. Here, we find that GapR, a chromosome structuring protein in Caulobacter crescentus, is required to complete DNA replication. GapR associates in vivo with positively supercoiled chromosomal DNA, and our biochemical and structural studies demonstrate that GapR forms a dimer-of-dimers that fully encircles overtwisted DNA. Further, we show that GapR stimulates gyrase and topo IV to relax positive supercoils, thereby enabling DNA replication. Analogous chromosome structuring proteins that locate to the overtwisted DNA in front of replication forks may be present in other organisms, similarly helping to recruit and stimulate topoisomerases during DNA replication.

PubMed: 30220456
DOI: 10.1016/j.cell.2018.08.029

実験手法

X-RAY DIFFRACTION (2 Å)