6CFB

Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti

6CFB の概要

• エントリーDOI: 10.2210/pdb6cfb/pdb
• NMR情報: BMRB: 30409
• 分子名称: barrettide A (1 entity in total)
• 機能のキーワード: structure from molmol, disulfide rich peptide, antifungal, marine sponge, antifungal protein
• 由来する生物種: Geodia barretti
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3220.46

構造登録者

Rosengren, K.J.,Carstens, B.B.,Clark, R.J.,Goransson, U. (登録日: 2018-02-14, 公開日: 2018-03-21, 最終更新日: 2024-11-06)

主引用文献

Carstens, B.B.,Rosengren, K.J.,Gunasekera, S.,Schempp, S.,Bohlin, L.,Dahlstrom, M.,Clark, R.J.,Goransson, U.
Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti.
J. Nat. Prod., 78:1886-1893, 2015

PubMed Abstract: Two disulfide-containing peptides, barrettides A (1) and B (2), from the cold-water marine sponge Geodia barretti are described. Those 31 amino acid residue long peptides were sequenced using mass spectrometry methods and structurally characterized using NMR spectroscopy. The structure of 1 was confirmed by total synthesis using the solid-phase peptide synthesis approach that was developed. The two peptides were found to differ only at a single position in their sequence. The three-dimensional structure of 1 revealed that these peptides possess a unique fold consisting of a long β-hairpin structure that is cross-braced by two disulfide bonds in a ladder-like arrangement. The peptides are amphipathic in nature with the hydrophobic and charged residues clustered on separate faces of the molecule. The barrettides were found not to inhibit the growth of either Escherichia coli or Staphylococcus aureus but displayed antifouling activity against barnacle larvae (Balanus improvisus) without lethal effects in the concentrations tested.

PubMed: 26222779
DOI: 10.1021/acs.jnatprod.5b00210

実験手法

SOLUTION NMR