6CF2

Crystal structure of HIV-1 Rev (residues 1-93)-RNA aptamer complex

6CF2 の概要

• エントリーDOI: 10.2210/pdb6cf2/pdb
• 分子名称: Anti-Rev Antibody, heavy chain, Anti-Rev Antibody, light chain, Protein Rev, ... (4 entities in total)
• 機能のキーワード: hiv-1, rev, rna aptamer, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47087.67

構造登録者

Eren, E.,Dearborn, A.D.,Wingfield, P.T. (登録日: 2018-02-13, 公開日: 2018-07-25, 最終更新日: 2024-10-16)

主引用文献

Dearborn, A.D.,Eren, E.,Watts, N.R.,Palmer, I.W.,Kaufman, J.D.,Steven, A.C.,Wingfield, P.T.
Structure of an RNA Aptamer that Can Inhibit HIV-1 by Blocking Rev-Cognate RNA (RRE) Binding and Rev-Rev Association.
Structure, 26:1187-, 2018

PubMed Abstract: HIV-1 Rev protein mediates nuclear export of unspliced and partially spliced viral RNAs for production of viral genomes and structural proteins. Rev assembles on a 351-nt Rev response element (RRE) within viral transcripts and recruits host export machinery. Small (<40-nt) RNA aptamers that compete with the RRE for Rev binding inhibit HIV-1 viral replication. We determined the X-ray crystal structure of a potential anti-HIV-1 aptamer that binds Rev with high affinity (K = 5.9 nM). The aptamer is structurally similar to the RRE high-affinity site but forms additional contacts with Rev unique to its sequence. Exposed bases of the aptamer interleave with the guanidinium groups of two arginines of Rev, forming stacking interactions and hydrogen bonds. The aptamer also obstructs an oligomerization interface of Rev, blocking Rev self-assembly. We propose that this aptamer can inhibit HIV-1 replication by interfering with Rev-RRE, Rev-Rev, and possibly Rev-host protein interactions.

PubMed: 30017564
DOI: 10.1016/j.str.2018.06.001

実験手法

X-RAY DIFFRACTION (3 Å)