6CA9

Crystal structure of Fab PCT64_LMCA (SAR), the least mutated common ancestor of the HIV-1 broadly neutralizing antibody lineage PCT64

6CA9 の概要

• エントリーDOI: 10.2210/pdb6ca9/pdb
• 関連するPDBエントリー: 5FEH 6AC6 6AC7
• 分子名称: PCT64_LMCA Fab light chain, PCT64_LMCA Fab heavy chain, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: igg1, antibody, hiv-1, bnab, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98221.03

構造登録者

Omorodion, O.,Wilson, I.A. (登録日: 2018-01-29, 公開日: 2018-06-27, 最終更新日: 2024-10-16)

主引用文献

Rantalainen, K.,Berndsen, Z.T.,Murrell, S.,Cao, L.,Omorodion, O.,Torres, J.L.,Wu, M.,Umotoy, J.,Copps, J.,Poignard, P.,Landais, E.,Paulson, J.C.,Wilson, I.A.,Ward, A.B.
Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design.
Cell Rep, 23:3249-3261, 2018

PubMed Abstract: Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system. Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort. Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes. Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers. These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.

PubMed: 29898396
DOI: 10.1016/j.celrep.2018.05.046

実験手法

X-RAY DIFFRACTION (2.702 Å)