6C9I

Single-Particle reconstruction of DARP14 - A designed protein scaffold displaying ~17kDa DARPin proteins - Scaffold

6C9I の概要

• エントリーDOI: 10.2210/pdb6c9i/pdb
• EMDBエントリー: 7403 7436 7436
• 分子名称: DARP14 - Subunit A with DARPin, DARP14 - Subunit B (2 entities in total)
• 機能のキーワード: designed complex, darpin, scaffold, single-particle cryo-em, de novo protein
• 由来する生物種: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3); 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 390906.46

構造登録者

Gonen, S.,Liu, Y.,Yeates, T.O.,Gonen, T. (登録日: 2018-01-26, 公開日: 2018-03-21, 最終更新日: 2024-03-13)

主引用文献

Liu, Y.,Gonen, S.,Gonen, T.,Yeates, T.O.
Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system.
Proc. Natl. Acad. Sci. U.S.A., 115:3362-3367, 2018

PubMed Abstract: Current single-particle cryo-electron microscopy (cryo-EM) techniques can produce images of large protein assemblies and macromolecular complexes at atomic level detail without the need for crystal growth. However, proteins of smaller size, typical of those found throughout the cell, are not presently amenable to detailed structural elucidation by cryo-EM. Here we use protein design to create a modular, symmetrical scaffolding system to make protein molecules of typical size suitable for cryo-EM. Using a rigid continuous alpha helical linker, we connect a small 17-kDa protein (DARPin) to a protein subunit that was designed to self-assemble into a cage with cubic symmetry. We show that the resulting construct is amenable to structural analysis by single-particle cryo-EM, allowing us to identify and solve the structure of the attached small protein at near-atomic detail, ranging from 3.5- to 5-Å resolution. The result demonstrates that proteins considerably smaller than the theoretical limit of 50 kDa for cryo-EM can be visualized clearly when arrayed in a rigid fashion on a symmetric designed protein scaffold. Furthermore, because the amino acid sequence of a DARPin can be chosen to confer tight binding to various other protein or nucleic acid molecules, the system provides a future route for imaging diverse macromolecules, potentially broadening the application of cryo-EM to proteins of typical size in the cell.

PubMed: 29507202
DOI: 10.1073/pnas.1718825115

実験手法

ELECTRON MICROSCOPY (3.09 Å)