6C80

Crystal structure of a flax cytokinin oxidase

6C80 の概要

• エントリーDOI: 10.2210/pdb6c80/pdb
• 分子名称: cytokinin oxidase LuCKX1.1, FLAVIN-ADENINE DINUCLEOTIDE, DI(HYDROXYETHYL)ETHER, ... (7 entities in total)
• 機能のキーワード: plant immunity, effector, cytokinin, cytokinin oxidase, immune system
• 由来する生物種: Linum usitatissimum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111961.83

構造登録者

Wan, L.,Williams, S.,Kobe, B. (登録日: 2018-01-23, 公開日: 2018-06-20, 最終更新日: 2024-11-06)

主引用文献

Wan, L.,Koeck, M.,Williams, S.J.,Ashton, A.R.,Lawrence, G.J.,Sakakibara, H.,Kojima, M.,Bottcher, C.,Ericsson, D.J.,Hardham, A.R.,Jones, D.A.,Ellis, J.G.,Kobe, B.,Dodds, P.N.
Structural and functional insights into the modulation of the activity of a flax cytokinin oxidase by flax rust effector AvrL567-A.
Mol. Plant Pathol., 20:211-222, 2019

PubMed Abstract: During infection, plant pathogens secrete effector proteins to facilitate colonization. In comparison with our knowledge of bacterial effectors, the current understanding of how fungal effectors function is limited. In this study, we show that the effector AvrL567-A from the flax rust fungus Melampsora lini interacts with a flax cytosolic cytokinin oxidase, LuCKX1.1, using both yeast two-hybrid and in planta bimolecular fluorescence assays. Purified LuCKX1.1 protein shows catalytic activity against both N6-(Δ2-isopentenyl)-adenine (2iP) and trans-zeatin (tZ) substrates. Incubation of LuCKX1.1 with AvrL567-A results in increased catalytic activity against both substrates. The crystal structure of LuCKX1.1 and docking studies with AvrL567-A indicate that the AvrL567 binding site involves a flexible surface-exposed region that surrounds the cytokinin substrate access site, which may explain its effect in modulating LuCKX1.1 activity. Expression of AvrL567-A in transgenic flax plants gave rise to an epinastic leaf phenotype consistent with hormonal effects, although no difference in overall cytokinin levels was observed. We propose that, during infection, plant pathogens may differentially modify the levels of extracellular and intracellular cytokinins.

PubMed: 30242946
DOI: 10.1111/mpp.12749

実験手法

X-RAY DIFFRACTION (1.78 Å)