6C7Z

Crystal structure of the Q108K:K40L:T51V:R58F mutant of human Cellular Retinol Binding Protein II in complex with synthetic Ligand Julolidine

Summary for 6C7Z

• Entry DOI: 10.2210/pdb6c7z/pdb
• Related: 5F58
• Descriptor: Retinol-binding protein 2, ACETATE ION, (2E,4E)-3-methyl-5-(2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)penta-2,4-dienal, ... (4 entities in total)
• Functional Keywords: protein engineering, ratiometric ph probe, hcrbpii, transport protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 2
• Total formula weight: 31793.80

Authors

Nosrati, M.,Geiger, J.H. (deposition date: 2018-01-23, release date: 2018-04-25, Last modification date: 2024-11-13)

Primary citation

Berbasova, T.,Tahmasebi Nick, S.,Nosrati, M.,Nossoni, Z.,Santos, E.M.,Vasileiou, C.,Geiger, J.H.,Borhan, B.
A Genetically Encoded Ratiometric pH Probe: Wavelength Regulation-Inspired Design of pH Indicators.
Chembiochem, 19:1288-1295, 2018

PubMed Abstract: Mutants of human cellular retinol-binding protein II (hCRBPII) were engineered to bind a julolidine retinal analogue for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, which affects the absorption and, thus, the emission of the protein/fluorophore complex. The ratio of emissions obtained at two excitation wavelengths that correspond to the absorption of the two forms of the protein/fluorophore complex, leads to a concentration-independent measure of pH.

PubMed: 29645331
DOI: 10.1002/cbic.201800050

Experimental method

X-RAY DIFFRACTION (1.42 Å)