6C2C

The molecular basis for the functional evolution of an organophosphate hydrolysing enzyme

6C2C の概要

• エントリーDOI: 10.2210/pdb6c2c/pdb
• 分子名称: dihydrocoumarin hydrolase, AncDHCH1, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: phosphatase, directed evolution, methyl-parathion hydrolase (mph), hydrolase, a xenobiotic degrading enzyme
• 由来する生物種: Pseudomonas sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63557.91

構造登録者

Hong, N.-S.,Jackson, C.J.,Carr, P.D.,Tokuriki, N.,Baier, F.,Yang, G. (登録日: 2018-01-08, 公開日: 2019-01-16, 最終更新日: 2024-03-13)

主引用文献

Yang, G.,Anderson, D.W.,Baier, F.,Dohmen, E.,Hong, N.,Carr, P.D.,Kamerlin, S.C.L.,Jackson, C.J.,Bornberg-Bauer, E.,Tokuriki, N.
Higher-order epistasis shapes the fitness landscape of a xenobiotic-degrading enzyme.
Nat.Chem.Biol., 15:1120-1128, 2019

PubMed Abstract: Characterizing the adaptive landscapes that encompass the emergence of novel enzyme functions can provide molecular insights into both enzymatic and evolutionary mechanisms. Here, we combine ancestral protein reconstruction with biochemical, structural and mutational analyses to characterize the functional evolution of methyl-parathion hydrolase (MPH), an organophosphate-degrading enzyme. We identify five mutations that are necessary and sufficient for the evolution of MPH from an ancestral dihydrocoumarin hydrolase. In-depth analyses of the adaptive landscapes encompassing this evolutionary transition revealed that the mutations form a complex interaction network, defined in part by higher-order epistasis, that constrained the adaptive pathways available. By also characterizing the adaptive landscapes in terms of their functional activities towards three additional organophosphate substrates, we reveal that subtle differences in the polarity of the substrate substituents drastically alter the network of epistatic interactions. Our work suggests that the mutations function collectively to enable substrate recognition via subtle structural repositioning.

PubMed: 31636435
DOI: 10.1038/s41589-019-0386-3

実験手法

X-RAY DIFFRACTION (1.597 Å)