6C1E

NavAb NormoPP mutant

6C1E の概要

• エントリーDOI: 10.2210/pdb6c1e/pdb
• 分子名称: Ion transport protein, 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, 5-BETA-24-NOR-CHOLANE-3(ALPHA),7(ALPHA),12(ALPHA)-TRIOL, ... (4 entities in total)
• 機能のキーワード: mutant, metal transport
• 由来する生物種: Arcobacter butzleri (strain RM4018)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79000.95

構造登録者

Catterall, W.A.,Zheng, N.,Jiang, D.,Gamal El-Din, T.M. (登録日: 2018-01-04, 公開日: 2018-05-16, 最終更新日: 2023-10-04)

主引用文献

Jiang, D.,Gamal El-Din, T.M.,Ing, C.,Lu, P.,Pomes, R.,Zheng, N.,Catterall, W.A.
Structural basis for gating pore current in periodic paralysis.
Nature, 557:590-594, 2018

PubMed Abstract: Potassium-sensitive hypokalaemic and normokalaemic periodic paralysis are inherited skeletal muscle diseases characterized by episodes of flaccid muscle weakness. They are caused by single mutations in positively charged residues ('gating charges') in the S4 transmembrane segment of the voltage sensor of the voltage-gated sodium channel Na1.4 or the calcium channel Ca1.1. Mutations of the outermost gating charges (R1 and R2) cause hypokalaemic periodic paralysis by creating a pathogenic gating pore in the voltage sensor through which cations leak in the resting state. Mutations of the third gating charge (R3) cause normokalaemic periodic paralysis owing to cation leak in both activated and inactivated states . Here we present high-resolution structures of the model bacterial sodium channel NaAb with the analogous gating-charge mutations, which have similar functional effects as in the human channels. The R2G and R3G mutations have no effect on the backbone structures of the voltage sensor, but they create an aqueous cavity near the hydrophobic constriction site that controls gating charge movement through the voltage sensor. The R3G mutation extends the extracellular aqueous cleft through the entire length of the activated voltage sensor, creating an aqueous path through the membrane. Conversely, molecular modelling shows that the R2G mutation creates a continuous aqueous path through the membrane only in the resting state. Crystal structures of NaAb(R2G) in complex with guanidinium define a potential drug target site. Molecular dynamics simulations illustrate the mechanism of Na permeation through the mutant gating pore in concert with conformational fluctuations of the gating charge R4. Our results reveal pathogenic mechanisms of periodic paralysis at the atomic level and suggest designs of drugs that may prevent ionic leak and provide symptomatic relief from hypokalaemic and normokalaemic periodic paralysis.

PubMed: 29769724
DOI: 10.1038/s41586-018-0120-4

実験手法

X-RAY DIFFRACTION (2.86 Å)