6C10

Crystal structure of mouse PCDH15 EC11-EL

6C10 の概要

• エントリーDOI: 10.2210/pdb6c10/pdb
• 分子名称: Protocadherin-15, alpha-D-mannopyranose (3 entities in total)
• 機能のキーワード: pcdh15, lhfpl5, protocadherin, tip link, hair cell, tmhs, hearing, membrane protein, metal transport
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28091.99

構造登録者

Gouaux, E.,Elferich, J.,Ge, J. (登録日: 2018-01-03, 公開日: 2018-08-15, 最終更新日: 2024-10-16)

主引用文献

Ge, J.,Elferich, J.,Goehring, A.,Zhao, J.,Schuck, P.,Gouaux, E.
Structure of mouse protocadherin 15 of the stereocilia tip link in complex with LHFPL5.
Elife, 7:-, 2018

PubMed Abstract: Hearing and balance involve the transduction of mechanical stimuli into electrical signals by deflection of bundles of stereocilia linked together by protocadherin 15 (PCDH15) and cadherin 23 'tip links'. PCDH15 transduces tip link tension into opening of a mechano-electrical transduction (MET) ion channel. PCDH15 also interacts with LHFPL5, a candidate subunit of the MET channel. Here we illuminate the PCDH15-LHFPL5 structure, showing how the complex is composed of PCDH15 and LHFPL5 subunit pairs related by a 2-fold axis. The extracellular cadherin domains define a mobile tether coupled to a rigid, 2-fold symmetric 'collar' proximal to the membrane bilayer. LHFPL5 forms extensive interactions with the PCDH15 transmembrane helices and stabilizes the overall PCDH15-LHFPL5 assembly. Our studies illuminate the architecture of the PCDH15-LHFPL5 complex, localize mutations associated with deafness, and shed new light on how forces in the PCDH15 tether may be transduced into the stereocilia membrane.

PubMed: 30070639
DOI: 10.7554/eLife.38770

実験手法

X-RAY DIFFRACTION (1.399 Å)