6BY4

Single-State 14-mer UUCG Tetraloop calculated from Exact NOEs

6BY4 の概要

• エントリーDOI: 10.2210/pdb6by4/pdb
• NMR情報: BMRB: 30386
• 分子名称: RNA (5'-R(P*GP*GP*CP*AP*CP*UP*UP*CP*GP*GP*UP*GP*CP*C)-3') (1 entity in total)
• 機能のキーワード: rna, exact noe, uucg tetraloop, 14-mer hairpin, single-state
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4454.68

構造登録者

Nichols, P.J.,Henen, M.A.,Born, A.,Strotz, D.,Guntert, P.,Vogeli, B. (登録日: 2017-12-19, 公開日: 2018-06-13, 最終更新日: 2024-05-15)

主引用文献

Nichols, P.J.,Henen, M.A.,Born, A.,Strotz, D.,Guntert, P.,Vogeli, B.
High-resolution small RNA structures from exact nuclear Overhauser enhancement measurements without additional restraints.
Commun Biol, 1:61-61, 2018

PubMed Abstract: RNA not only translates the genetic code into proteins, but also carries out important cellular functions. Understanding such functions requires knowledge of the structure and dynamics at atomic resolution. Almost half of the published RNA structures have been solved by nuclear magnetic resonance (NMR). However, as a result of severe resonance overlap and low proton density, high-resolution RNA structures are rarely obtained from nuclear Overhauser enhancement (NOE) data alone. Instead, additional semi-empirical restraints and labor-intensive techniques are required for structural averages, while there are only a few experimentally derived ensembles representing dynamics. Here we show that our exact NOE (eNOE) based structure determination protocol is able to define a 14-mer UUCG tetraloop structure at high resolution without other restraints. Additionally, we use eNOEs to calculate a two-state structure, which samples its conformational space. The protocol may open an avenue to obtain high-resolution structures of small RNA of unprecedented accuracy with moderate experimental efforts.

PubMed: 30271943
DOI: 10.1038/s42003-018-0067-x

実験手法

SOLUTION NMR