6BY1

E. coli pH03H9 complex

これはPDB形式変換不可エントリーです。

6BY1 の概要

• エントリーDOI: 10.2210/pdb6by1/pdb
• 分子名称: 16S ribosomal RNA, 50S ribosomal protein L9, 50S ribosomal protein L10, ... (58 entities in total)
• 機能のキーワード: mrna, trna, helicase, ribosome
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 112
• 化学式量合計: 4393633.60

構造登録者

Amiri, H.,Noller, H.F. (登録日: 2017-12-19, 公開日: 2019-02-27, 最終更新日: 2024-11-06)

主引用文献

Amiri, H.,Noller, H.F.
Structural evidence for product stabilization by the ribosomal mRNA helicase.
Rna, 25:364-375, 2019

PubMed Abstract: Protein synthesis in all organisms proceeds by stepwise translocation of the ribosome along messenger RNAs (mRNAs), during which the helicase activity of the ribosome unwinds encountered structures in the mRNA. This activity is known to occur near the mRNA tunnel entrance, which is lined by ribosomal proteins uS3, uS4, and uS5. However, the mechanism(s) of mRNA unwinding by the ribosome and the possible role of these proteins in the helicase activity are not well understood. Here, we present a crystal structure of the ribosome in which single-stranded mRNA is observed beyond the tunnel entrance, interacting in an extended conformation with a positively charged patch on ribosomal protein uS3 immediately outside the entrance. This apparent binding specificity for single-stranded mRNA ahead of the tunnel entrance suggests that product stabilization may play a role in the unwinding of structured mRNA by the ribosomal helicase.

PubMed: 30552154
DOI: 10.1261/rna.068965.118

実験手法

X-RAY DIFFRACTION (3.94 Å)