6BUG

Crystal structure of a membrane protein, crystal form I

6BUG の概要

• エントリーDOI: 10.2210/pdb6bug/pdb
• 分子名称: D-alanyl carrier protein, D-alanyl transfer protein DltB (2 entities in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Streptococcus thermophilus; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 228781.26

構造登録者

Ma, D.,Wang, Z.,Xu, W. (登録日: 2017-12-10, 公開日: 2018-10-10, 最終更新日: 2024-10-30)

主引用文献

Ma, D.,Wang, Z.,Merrikh, C.N.,Lang, K.S.,Lu, P.,Li, X.,Merrikh, H.,Rao, Z.,Xu, W.
Crystal structure of a membrane-bound O-acyltransferase.
Nature, 562:286-290, 2018

PubMed Abstract: Membrane-bound O-acyltransferases (MBOATs) are a superfamily of integral transmembrane enzymes that are found in all kingdoms of life. In bacteria, MBOATs modify protective cell-surface polymers. In vertebrates, some MBOAT enzymes-such as acyl-coenzyme A:cholesterol acyltransferase and diacylglycerol acyltransferase 1-are responsible for lipid biosynthesis or phospholipid remodelling. Other MBOATs, including porcupine, hedgehog acyltransferase and ghrelin acyltransferase, catalyse essential lipid modifications of secreted proteins such as Wnt, hedgehog and ghrelin, respectively. Although many MBOAT proteins are important drug targets, little is known about their molecular architecture and functional mechanisms. Here we present crystal structures of DltB, an MBOAT responsible for the D-alanylation of cell-wall teichoic acid in Gram-positive bacteria, both alone and in complex with the D-alanyl donor protein DltC. DltB contains a ring of 11 peripheral transmembrane helices, which shield a highly conserved extracellular structural funnel extending into the middle of the lipid bilayer. The conserved catalytic histidine residue is located at the bottom of this funnel and is connected to the intracellular DltC through a narrow tunnel. Mutation of either the catalytic histidine or the DltC-binding site of DltB abolishes the D-alanylation of lipoteichoic acid and sensitizes the Gram-positive bacterium Bacillus subtilis to cell-wall stress, which suggests cross-membrane catalysis involving the tunnel. Structure-guided sequence comparison among DltB and vertebrate MBOATs reveals a conserved structural core and suggests that MBOATs from different organisms have similar catalytic mechanisms. Our structures provide a template for understanding structure-function relationships in MBOATs and for developing therapeutic MBOAT inhibitors.

PubMed: 30283133
DOI: 10.1038/s41586-018-0568-2

実験手法

X-RAY DIFFRACTION (3.27 Å)