6BTE

DNA Polymerase Beta I260Q Binary Complex

6BTE の概要

• エントリーDOI: 10.2210/pdb6bte/pdb
• 分子名称: DNA polymerase beta, DNA Template Strand, DNA Primer Strand, ... (8 entities in total)
• 機能のキーワード: lyase, dna complex, transferase, lyase-dna complex, lyase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48042.08

構造登録者

Eckenroth, B.E.,Doublie, S. (登録日: 2017-12-06, 公開日: 2018-09-26, 最終更新日: 2023-10-04)

主引用文献

Liptak, C.,Mahmoud, M.M.,Eckenroth, B.E.,Moreno, M.V.,East, K.,Alnajjar, K.S.,Huang, J.,Towle-Weicksel, J.B.,Doublie, S.,Loria, J.P.,Sweasy, J.B.
I260Q DNA polymerase beta highlights precatalytic conformational rearrangements critical for fidelity.
Nucleic Acids Res., 46:10740-10756, 2018

PubMed Abstract: DNA polymerase β (pol β) fills single nucleotide gaps in DNA during base excision repair and non-homologous end-joining. Pol β must select the correct nucleotide from among a pool of four nucleotides with similar structures and properties in order to maintain genomic stability during DNA repair. Here, we use a combination of X-ray crystallography, fluorescence resonance energy transfer and nuclear magnetic resonance to show that pol β's ability to access the appropriate conformations both before and upon binding to nucleotide substrates is integral to its fidelity. Importantly, we also demonstrate that the inability of the I260Q mutator variant of pol β to properly navigate this conformational landscape results in error-prone DNA synthesis. Our work reveals that precatalytic conformational rearrangements themselves are an important underlying mechanism of substrate selection by DNA pol β.

PubMed: 30239932
DOI: 10.1093/nar/gky825

実験手法

X-RAY DIFFRACTION (2.2 Å)