6BTC

SCCmec type IV LP1413 - nucleic acids binding protein

6BTC の概要

• エントリーDOI: 10.2210/pdb6btc/pdb
• 分子名称: LP1413 - SCCmec type IV-encoded DNA binding protein (2 entities in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21964.91

構造登録者

Rice, P.A.,Mir-Sanchis, I. (登録日: 2017-12-06, 公開日: 2018-07-25, 最終更新日: 2024-10-09)

主引用文献

Mir-Sanchis, I.,Pigli, Y.Z.,Rice, P.A.
Crystal Structure of an Unusual Single-Stranded DNA-Binding Protein Encoded by Staphylococcal Cassette Chromosome Elements.
Structure, 26:1144-, 2018

PubMed Abstract: Methicillin-resistant Staphylococcus aureus is a global public health threat. Methicillin resistance is carried on mobile genetic elements belonging to the staphylococcal cassette chromosome (SCC) family. The molecular mechanisms that SCC elements exploit for stable maintenance and for horizontal transfer are poorly understood. Previously, we identified several conserved SCC genes with putative functions in DNA replication, including lp1413, which we found encodes a single-stranded DNA (ssDNA)-binding protein. We report here the 2.18 Å crystal structure of LP1413, which shows that it adopts a winged helix-turn-helix fold rather than the OB-fold normally seen in replication-related ssDNA-binding proteins. However, conserved residues form a hydrophobic pocket not normally found in winged helix-turn-helix domains. LP1413 also has a conserved but disordered C-terminal tail. As deletion of the tail does not significantly affect cooperative binding to ssDNA, we propose that it mediates interactions with other proteins. LP1413 could play several different roles in vivo.

PubMed: 30017563
DOI: 10.1016/j.str.2018.05.016

実験手法

X-RAY DIFFRACTION (2.17740739208 Å)