6BRA

HIV-1 protease (D25N, inactive) in complex with phage display optimized substrate SGIFLETS

6BRA の概要

• エントリーDOI: 10.2210/pdb6bra/pdb
• 分子名称: Protease, Phage display-optimized HIV-1 protease substrate, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: hiv, hiv-1, substrate, complex, phage display, recongition, hydrogen bonding, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 22474.12

構造登録者

Windsor, I.W.,Raines, R.T. (登録日: 2017-11-30, 公開日: 2018-07-18, 最終更新日: 2023-10-04)

主引用文献

Windsor, I.W.,Raines, R.T.
A substrate selected by phage display exhibits enhanced side-chain hydrogen bonding to HIV-1 protease.
Acta Crystallogr D Struct Biol, 74:690-694, 2018

PubMed Abstract: Crystal structures of inactive variants of HIV-1 protease bound to peptides have revealed how the enzyme recognizes its endogenous substrates. The best of the known substrates is, however, a nonnatural substrate that was identified by directed evolution. The crystal structure of the complex between this substrate and the D25N variant of the protease is reported at a resolution of 1.1 Å. The structure has several unprecedented features, especially the formation of additional hydrogen bonds between the enzyme and the substrate. This work expands the understanding of molecular recognition by HIV-1 protease and informs the design of new substrates and inhibitors.

PubMed: 29968678
DOI: 10.1107/S2059798318006691

実験手法

X-RAY DIFFRACTION (1.111 Å)