6BQO

Structure of a dual topology fluoride channel with monobody S8

6BQO の概要

• エントリーDOI: 10.2210/pdb6bqo/pdb
• 分子名称: Fluoride ion transporter CrcB, Monobody S8, FLUORIDE ION, ... (6 entities in total)
• 機能のキーワード: fluoride channel, monobody, dual topology, membrane protein
• 由来する生物種: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 38097.34

構造登録者

Stockbridge, R.B.,Newstead, S.,McIlwain, B.C. (登録日: 2017-11-28, 公開日: 2018-03-07, 最終更新日: 2023-10-04)

主引用文献

McIlwain, B.C.,Newstead, S.,Stockbridge, R.B.
Cork-in-Bottle Occlusion of Fluoride Ion Channels by Crystallization Chaperones.
Structure, 26:635-639.e1, 2018

PubMed Abstract: Crystallization of dual-topology fluoride (Fluc) channels requires small, soluble crystallization chaperones known as monobodies, which act as primary crystal lattice contacts. Previous structures of Flucs have been solved in the presence of monobodies that inhibit fluoride currents in single-channel electrophysiological recordings. These structures have revealed two-fold symmetric, doubly bound arrangements, with one monobody on each side of the membrane. The combined electrophysiological and structural observations raise the possibility that chaperone binding allosterically closes the channel, altering the structure from its conducting form. To address this, we identify and solve the structure with a different monobody that only partially blocks fluoride currents. The structure of the channel-monobody complex is asymmetric, with monobody bound to one side of the channel only. The channel conformation is nearly identical on the bound and uncomplexed sides, and to all previously solved structures, providing direct structural evidence that monobody binding does not induce local structural changes.

PubMed: 29526432
DOI: 10.1016/j.str.2018.02.004

実験手法

X-RAY DIFFRACTION (2.8 Å)