6BQ1

Human PI4KIIIa lipid kinase complex

6BQ1 の概要

• エントリーDOI: 10.2210/pdb6bq1/pdb
• EMDBエントリー: 7129
• 分子名称: Phosphatidylinositol 4-kinase III alpha (PI4KA), Tetratricopeptide repeat protein 7B, Protein FAM126A, ... (5 entities in total)
• 機能のキーワード: kinase, phosphoinositide synthesis, transferase-signaling protein complex, transferase/signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 607175.40

構造登録者

Lees, J.A.,Zhang, Y.,Oh, M.,Schauder, C.M.,Yu, X.,Baskin, J.,Dobbs, K.,Notarangelo, L.D.,Camilli, P.D.,Walz, T.,Reinisch, K.M. (登録日: 2017-11-27, 公開日: 2017-12-13, 最終更新日: 2024-03-13)

主引用文献

Lees, J.A.,Zhang, Y.,Oh, M.S.,Schauder, C.M.,Yu, X.,Baskin, J.M.,Dobbs, K.,Notarangelo, L.D.,De Camilli, P.,Walz, T.,Reinisch, K.M.
Architecture of the human PI4KIII alpha lipid kinase complex.
Proc. Natl. Acad. Sci. U.S.A., 114:13720-13725, 2017

PubMed Abstract: Plasma membrane (PM) phosphoinositides play essential roles in cell physiology, serving as both markers of membrane identity and signaling molecules central to the cell's interaction with its environment. The first step in PM phosphoinositide synthesis is the conversion of phosphatidylinositol (PI) to PI4P, the precursor of PI(4,5)P and PI(3,4,5)P This conversion is catalyzed by the PI4KIIIα complex, comprising a lipid kinase, PI4KIIIα, and two regulatory subunits, TTC7 and FAM126. We here report the structure of this complex at 3.6-Å resolution, determined by cryo-electron microscopy. The proteins form an obligate ∼700-kDa superassembly with a broad surface suitable for membrane interaction, toward which the kinase active sites are oriented. The structural complexity of the assembly highlights PI4P synthesis as a major regulatory junction in PM phosphoinositide homeostasis. Our studies provide a framework for further exploring the mechanisms underlying PM phosphoinositide regulation.

PubMed: 29229838
DOI: 10.1073/pnas.1718471115

実験手法

ELECTRON MICROSCOPY (3.6 Å)