6BPM

The crystal structure of the Ferric-Catecholate import receptor Fiu from K12 E. coli: Closed form (C21)

6BPM の概要

• エントリーDOI: 10.2210/pdb6bpm/pdb
• 分子名称: Catecholate siderophore receptor Fiu, (20S)-2,5,8,11,14,17-HEXAMETHYL-3,6,9,12,15,18-HEXAOXAHENICOSANE-1,20-DIOL, octyl beta-D-glucopyranoside, ... (4 entities in total)
• 機能のキーワード: ferric-catecholate, tonb-dependent receptor, membrane transport, antibiotic uptake, iron transporter, membrane protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 159963.07

構造登録者

Grinter, R. (登録日: 2017-11-23, 公開日: 2018-11-28, 最終更新日: 2024-11-06)

主引用文献

Grinter, R.,Lithgow, T.
The structure of the bacterial iron-catecholate transporter Fiu suggests that it imports substrates via a two-step mechanism.
J.Biol.Chem., 2019

PubMed Abstract: The ferric iron uptake (Fiu) transporter from functions in the transport of iron-catecholate complexes across the bacterial outer membrane, providing the bacterium with iron, which is essential for growth. Recently it has become clear that Fiu also represents a liability for because its activity allows import of antimicrobial compounds that mimic catecholate. This inadvertent import suggests the potential utility of antimicrobial catechol siderophore mimetics in managing bacterial infections. However, to fully exploit these compounds, a detailed understanding of the mechanism of transport through Fiu and related transporters is required. To address this question, we determined the crystal structure of Fiu at 2.1-2.9 Å and analyzed its function in Through analysis of the Fiuo crystal structure, in combination with docking and mutagenesis, we provide insight into how Fiu and related transporters bind catecholate in a surface-exposed cavity. Moreover, through determination of the structure of Fiu in multiple crystal states, we revealed the presence of a large, selectively gated cavity in the interior of this transporter. This chamber is large enough to accommodate the Fiu substrate and may allow import of substrates via a two-step mechanism. This would avoid channel formation through the transporter and inadvertent import of toxic molecules. As Fiu and its homologs are the targets of substrate-mimicking antibiotics, these results may assist in the development of these compounds.

PubMed: 31712312
DOI: 10.1074/jbc.RA119.011018

実験手法

X-RAY DIFFRACTION (2.5 Å)