6BPH

Crystal structure of the chromodomain of RBBP1

6BPH の概要

• エントリーDOI: 10.2210/pdb6bph/pdb
• 分子名称: AT-rich interactive domain-containing protein 4A, UNKNOWN ATOM OR ION (3 entities in total)
• 機能のキーワード: crystal structure of the chromodomain of rbbp1, structural genomics, structural genomics consortium, sgc, unknown function
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus : P29374
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8068.16

構造登録者

Liu, Y.,Tempel, W.,Bountra, C.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2017-11-23, 公開日: 2017-12-20, 最終更新日: 2024-11-06)

主引用文献

Lei, M.,Feng, Y.,Zhou, M.,Yang, Y.,Loppnau, P.,Li, Y.,Yang, Y.,Liu, Y.
Crystal structure of chromo barrel domain of RBBP1.
Biochem. Biophys. Res. Commun., 496:1344-1348, 2018

PubMed Abstract: RBBP1 is a retinoblastoma protein (pRb) binding protein acting as a repressor of gene transcription. RBBP1 is a multidomain protein including a chromo barrel domain, and its chromo barrel domain has been reported to recognize histone H4K20me3 weakly, and this binding is enhanced by the simultaneous binding of DNA. However, the molecular basis of this DNA-mediated histone binding by the chromo barrel domain of RBBP1 is unclear. Here we attempted to co-crystallize the chromo barrel domain of RBBP1 with either a histone H4K20me3 peptide alone or with both a histone H4K20me3 peptide and DNA, but only solved the peptide/DNA unbound crystal structure. Our structural analysis indicates that RBBP1 could interact with histone H4K20me3 similar to other histone binding chromo barrel domains, and the surface charge representation analysis of the chromo barrel domain of RBBP1 suggests that the chromo barrel domain of RBBP1 does not have a typical DNA binding surface, indicating that it might not bind to DNA. Consistently, our ITC assays also showed that DNA does not significantly enhance the histone binding ability of the chromo barrel domain of RBBP1.

PubMed: 29408527
DOI: 10.1016/j.bbrc.2018.02.016

実験手法

X-RAY DIFFRACTION (1.85 Å)