6BN1

Salvador Hippo SARAH domain complex

6BN1 の概要

• エントリーDOI: 10.2210/pdb6bn1/pdb
• 分子名称: Serine/threonine-protein kinase hippo, Scaffold protein salvador, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• 機能のキーワード: hippo, signaling protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• 細胞内の位置: Apical cell membrane : Q8T0S6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15801.93

構造登録者

Cairns, L.,Kavran, J.M. (登録日: 2017-11-15, 公開日: 2018-03-14, 最終更新日: 2024-10-09)

主引用文献

Cairns, L.,Tran, T.,Fowl, B.H.,Patterson, A.,Kim, Y.J.,Bothner, B.,Kavran, J.M.
Salvador has an extended SARAH domain that mediates binding to Hippo kinase.
J. Biol. Chem., 293:5532-5543, 2018

PubMed Abstract: The Hippo pathway controls cell proliferation and differentiation through the precisely tuned activity of a core kinase cassette. The activity of Hippo kinase is modulated by interactions between its C-terminal coiled-coil, termed the SARAH domain, and the SARAH domains of either dRassF or Salvador. Here, we wanted to understand the molecular basis of SARAH domain-mediated interactions and their influence on Hippo kinase activity. We focused on Salvador, a positive effector of Hippo activity and the least well-characterized SARAH domain-containing protein. We determined the crystal structure of a complex between Salvador and Hippo SARAH domains from This structure provided insight into the organization of the Salvador SARAH domain including a folded N-terminal extension that expands the binding interface with Hippo SARAH domain. We also found that this extension improves the solubility of the Salvador SARAH domain, enhances binding to Hippo, and is unique to Salvador. We therefore suggest expanding the definition of the Salvador SARAH domain to include this extended region. The heterodimeric assembly observed in the crystal was confirmed by cross-linked MS and provided a structural basis for the mutually exclusive interactions of Hippo with either dRassF or Salvador. Of note, Salvador influenced the kinase activity of Mst2, the mammalian Hippo homolog. In co-transfected HEK293T cells, human Salvador increased the levels of Mst2 autophosphorylation and Mst2-mediated phosphorylation of select substrates, whereas Salvador SARAH domain inhibited Mst2 autophosphorylation These results suggest Salvador enhances the effects of Hippo kinase activity at multiple points in the Hippo pathway.

PubMed: 29519817
DOI: 10.1074/jbc.RA117.000923

実験手法

X-RAY DIFFRACTION (2.6 Å)