6BIO

Structure of NlpC1 from Trichomonas vaginalis

6BIO の概要

• エントリーDOI: 10.2210/pdb6bio/pdb
• 分子名称: Clan CA, family C40, NlpC/P60 superfamily cysteine peptidase (2 entities in total)
• 機能のキーワード: peptidase, nlpc protein, hydrolase
• 由来する生物種: Trichomonas vaginalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31656.55

構造登録者

Pinheiro, J.,Simoes-Barbosa, A.,Goldstone, D.C. (登録日: 2017-11-02, 公開日: 2018-11-07, 最終更新日: 2023-10-04)

主引用文献

Pinheiro, J.,Biboy, J.,Vollmer, W.,Hirt, R.P.,Keown, J.R.,Artuyants, A.,Black, M.M.,Goldstone, D.C.,Simoes-Barbosa, A.
The ProtozoanTrichomonas vaginalisTargets Bacteria with Laterally Acquired NlpC/P60 Peptidoglycan Hydrolases.
Mbio, 9:-, 2018

PubMed Abstract: The human eukaryotic pathogen causes trichomoniasis, a prevalent sexually transmitted infection. This extracellular protozoan is intimately associated with the human vaginal mucosa and microbiota, but key aspects of the complex interactions between the parasite and the vaginal bacteria remain elusive. We report that has acquired, by lateral gene transfer from bacteria, genes encoding peptidoglycan hydrolases of the NlpC/P60 family. Two of the enzymes were active against bacterial peptidoglycan, retaining the active-site fold and specificity as dl-endopeptidases. The endogenous NlpC/P60 genes are transcriptionally upregulated in in the presence of bacteria. The overexpression of an exogenous copy enables the parasite to outcompete bacteria from mixed cultures, consistent with the biochemical activity of the enzyme. Our study results highlight the relevance of the interactions of this eukaryotic pathogen with bacteria, a poorly understood aspect of the biology of this important human parasite. is a parasitic protozoan of the human urogenital tract that causes trichomoniasis, a very common sexually transmitted disease. Despite residing extracellularly and in close association with the vaginal bacteria (i.e., the microbiota), very little is known about the nature of the parasite-bacterium interactions. Our study showed that this parasite had acquired genes from bacteria which retained their original function. They produce active enzymes capable of degrading peptidoglycan, a unique polymer of the bacterial cell envelope, helping the parasite to outcompete bacteria in mixed cultures. This study was the first to show that a laterally acquired group of genes enables a eukaryotic mucosal pathogen to control bacterial population. We highlight the importance of understanding the interactions between pathogens and microbiota, as the outcomes of these interactions are increasingly understood to have important implications on health and disease.

PubMed: 30538181
DOI: 10.1128/mBio.01784-18

実験手法

X-RAY DIFFRACTION (1.2 Å)